Journal: Future science OA

Article Title: Adverse predictive value of ASPM on lung adenocarcinoma overall survival depended on chemotherapy status.

doi: 10.1080/20565623.2025.2489328

Figure Lengend Snippet: Figure 1. (A) TCGA TARGET GTEx pan-cancer dataset analysis showed the relationship between ASPM gene expression and patient prognosis in each tumor. (B) In TCGA dataset of LUAD, high ASPM expression was related to worse overall survival (Log-rank test p = 2.4e-6). (C) In proteomics dataset of LUAD, the difference of prognosis was not significant between ASPM high or low expression groups (Log-rank test p = 0.26).

Article Snippet: Equal amounts of protein were run in 10% SdS-paGE gel and transferred to a polyvinylidene difluoride (pVdF) membrane and then incubated with the primary antibodies at 4 °C overnight. the antibodies included anti-aSpm rabbit polyclonal antibody (1:1000, a02584-1, Boster, China), mouse monoclonal [mabcam 8226] to beta actin - Loading Control (1:1000, aB8226, abcam, uSa). after incubation with secondary antibody (1:2000; Santa Cruz), the signals were visualized and analyzed through image acquisition and analysis software (Bio-Rad, Hercules, Ca, uSa).

Techniques: Gene Expression, Expressing

Journal: Future science OA

Article Title: Adverse predictive value of ASPM on lung adenocarcinoma overall survival depended on chemotherapy status.

doi: 10.1080/20565623.2025.2489328

Figure Lengend Snippet: Figure 2. (A) The representative immunohistochemical staining of ASPM negative, weak, moderate, and strong cases. (B) In our cohort, high ASPM expression predicted better LUAD prognosis (Log-rank test p = 5.4e-4). (C) In patients who received chemotherapy, high ASPM expression was related to better overall survival (Log-rank test p = 5.0e-4). (D) In patients who did not received chemotherapy, ASPM expression was not related to patient overall survival (Log-rank test p = 0.48). (E) Multivariate regression analysis showed ASPM was not an independent prognostic factor while other protective factor included chemother apy and risk factor included clinical stages. (F) time dependent ROC analysis was conducted at time points 365, 1095, and 1825 days. The AUC and confidence intervals were evaluated using the ci function of pROC.

Article Snippet: Equal amounts of protein were run in 10% SdS-paGE gel and transferred to a polyvinylidene difluoride (pVdF) membrane and then incubated with the primary antibodies at 4 °C overnight. the antibodies included anti-aSpm rabbit polyclonal antibody (1:1000, a02584-1, Boster, China), mouse monoclonal [mabcam 8226] to beta actin - Loading Control (1:1000, aB8226, abcam, uSa). after incubation with secondary antibody (1:2000; Santa Cruz), the signals were visualized and analyzed through image acquisition and analysis software (Bio-Rad, Hercules, Ca, uSa).

Techniques: Immunohistochemical staining, Staining, Expressing

Journal: Future science OA

Article Title: Adverse predictive value of ASPM on lung adenocarcinoma overall survival depended on chemotherapy status.

doi: 10.1080/20565623.2025.2489328

Figure Lengend Snippet: Figure 3. (A) heatmap of signature proteins in ASPM low or high groups from proteomics dataset. (B) High ASPM1 related signature proteins were enriched mainly in cell cycle and mitosis processes by Metascape analysis. (C) ASPM positive group in proteomics dataset showed higher expression of proliferation related proteins mainly including AURKB, PLK1, KIFC3, EZH2,CDKN2A, BRD3, CASP2, MKI67, CDK1, and MDC1.

Article Snippet: Equal amounts of protein were run in 10% SdS-paGE gel and transferred to a polyvinylidene difluoride (pVdF) membrane and then incubated with the primary antibodies at 4 °C overnight. the antibodies included anti-aSpm rabbit polyclonal antibody (1:1000, a02584-1, Boster, China), mouse monoclonal [mabcam 8226] to beta actin - Loading Control (1:1000, aB8226, abcam, uSa). after incubation with secondary antibody (1:2000; Santa Cruz), the signals were visualized and analyzed through image acquisition and analysis software (Bio-Rad, Hercules, Ca, uSa).

Techniques: Expressing

Journal: Future science OA

Article Title: Adverse predictive value of ASPM on lung adenocarcinoma overall survival depended on chemotherapy status.

doi: 10.1080/20565623.2025.2489328

Figure Lengend Snippet: Figure 4. (A) Proteomics data analysis showed ASPM positive group had higher MKI67 expression. (B) Western blot analysis of A549 cells and H1299 cells confirmed effectiveness of ASPM transfection and in vitro overexpression. (C-D) Statistical anal ysis of western blot images. (E-F) CCK8 assay proved overexpression of ASPM promoted tumor cell proliferation in A549 and H1299 cells. (G-H) When treated with cisplatin, overexpression of ASPM enhanced the lethality on A549 and H1299 tumor cells. (P value *<0.05, **<0.01, ***<0.001).

Article Snippet: Equal amounts of protein were run in 10% SdS-paGE gel and transferred to a polyvinylidene difluoride (pVdF) membrane and then incubated with the primary antibodies at 4 °C overnight. the antibodies included anti-aSpm rabbit polyclonal antibody (1:1000, a02584-1, Boster, China), mouse monoclonal [mabcam 8226] to beta actin - Loading Control (1:1000, aB8226, abcam, uSa). after incubation with secondary antibody (1:2000; Santa Cruz), the signals were visualized and analyzed through image acquisition and analysis software (Bio-Rad, Hercules, Ca, uSa).

Techniques: Expressing, Western Blot, Transfection, In Vitro, Over Expression, CCK-8 Assay